衰老是生物体的结构与功能随生存时间发生的系统性衰退过程。人类的衰老过程伴随着衰老相关疾病及癌症等发生率的升高。对衰老过程的研究将为相关疾病的预防和治疗提供理论基础。染色质的表观遗传修饰调控基因组DNA参与的所有生物学过程。本实验室主要以线虫(Caenorhabditis elegans)为模式生物,通过生物化学、遗传学、生物信息学等方法研究衰老过程及物种寿命决定的调控机制。具体研究方向包括衰老过程中的染色质组分的代谢调控,及其在基因转录调控、DNA损伤修复等衰老相关过程中的功能。
Pu M, Lee SS. Chromatin Immunoprecipitation from Caenorhabditis elegans Somatic Cells. Methods Mol Biol. 2020;2144:171-175. doi:10.1007/978-1-0716-0592-9_15. PMID: 32410034.
贾梅,浦敏铁(2019)组蛋白修饰调控衰老过程的机制研究进展 中国科学: 生命科学 49: 806-813
Mintie Pu#, Minghui Wang, Wenke Wang and Siu Sylvia Lee# (2018) Unique patterns of trimethylation of histone H3 lysine 4 are prone to changes during aging in Caenorhabditis elegans somatic cells. PLoS Genet 14(6): e1007466. https://doi.org/10.1371/journal.pgen.1007466 (# co-corresponding author)
Mintie Pu, Zhuoyu Ni, Xiujuan Wang, Minghui Wang, Jason G. Wood, Stephen L. Helfand, Haiyuan Yu, and Siu Sylvia Lee (2015) Tri-methylation of lysine 36 on H3 restricts gene expression change during aging and impacts life span. Genes Dev. 29: 718-731
Recommended in F1000Prime as being of special significance in its field in 2017
Bin Kang*, Mintie Pu*, Weihong Wen, Zigang Dong, Bruce Stillman, and Zhiguo Zhang (2011) Phosphorylation of H4 Ser 47 promotes HIRA-mediated nucleosome assembly. Genes Dev. 25, 1359-64. (* authors contributed equally)
Jian Yuan, Mintie Pu, Zhiguo Zhang and Zhenkun Lou (2009) Histone H3-K56 acetylation is important for genomic stability in mammals. Cell Cycle 8, 1747-1753.
Jing-Yu Li*, Min-Tie Pu*, Ryutaro Hirasawa, Bing-Zhong Li, Yan-Nv Huang, Rong Zeng, Nai-He Jing, Taiping Chen, En Li, Hiroyuki Sasaki & Guo-Liang Xu (2007) Synergistic Function of DNA Methyltransferases Dnmt3a and Dnmt3b in the Methylation of Oct4 and Nanog. Mol. Cell. Biol. 27, 8748-59. (* authors contributed equally)
Ying-Zi Ge, Min-Tie Pu, Humaira Gowher, Hai-Ping Wu, Jian-Ping Ding, Albert Jeltsch, Guo-Liang Xu (2004) Chromatin Targeting of de Novo DNA Methyltransferases by the PWWP Domain. J. Biol. Chem. 279, 25447–25454.
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Email: mtpu37@ynu.edu.cn